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I.S07.4446 (Ds Insertion Line)    View RefGen_v1 Placement     Admin

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diagram of ds insertion

Predicted Ds insertion site / closest B73 gene for I.S07.4446       Order Seed    View all Ds Insertions

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Loc
Predicted Ds insertion site   Closest Maize Gene
GDB Ds Distance from Gene All Ds
near
gene
Flanking sequence (fDs)
Quality
Score
Seq
Pos
1 7: 122,070,623 GRMZM2G086714_T01
[View Locus Details]
0.2 kb from 3` end gi|226938842 (I.S07.4446_JSR03) MQ4 5a

Placement Details

More on Insertion Site...


This seedstock is available from the Maize Genetics Coop as AcDs-00632

To request seed of I.S07.4446 (AcDs-00632), you can add it to a Maize Coop Stock Center order -- this action takes you to the maizegdb.org website. Then complete the order on maizegdb, or use the "back" button to return to PlantGDB and additional searches. Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order (see below).

Before You Order...


Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 226938842 (I.S07.4446_JSR03) Blast@ZmGDB

Show FASTA Sequence for gi|226938842 (700 bp)

>gi|226938842|gb|FI849811|I.S07.4446
TTTAAAAGTGTTCCGAGTGATTCTAGACTATATCCCACCTGAAGCGGACACCGCTAGTTTCTTGTCCACATAGCCAGGCATGCCTGGCCGCCCTGGAGAC
ACGGACACCACGAGCAACCCTGATGTCACGGCTTCGCTCAAAGAAGCCGCCCATTTGGCGTAGCATCACAAGATCTAGTCATCCAAAAGGCGATGCAGAA
TTGCAGATGCAGCTACAGCTCTCTCCACAACAACAACAACAACAACAACAACAACAAAACTTGGGAGCAGCTGAAACGAACCTGAACAGCTTATATATAC
ACAGCGTTCTACCTTTACACATTTGCAGCTTCAGGCTGCAAGACCTAGCTAGCTTATACTTACAACGAATTGAATGGTACAATACAATGGTAGGAAAGGT
TAACATCATCGTATTGCTACTGTCAGCAGCGTCTCGAGCCACCGCCTTGAGCTGAGAGCGCGAAGCCGCCACGGTCGTACATGCGCTGGATCTCTTCCCG
GTACCCGCTGAGGGACCTGCGAGGCAGGGCCGGCGTCAGCTCCACCACGTCCCCCATGCTCAGCTTCCGGTTCGGGTCGCATATAGGCTCGTGGTTTACC
CGCGGCCGCAGATCCTCCTTCACGGGGATGCTGTAGGGGCTCCATCTCGAGCTGTTGGCGCCGACACGGTCCATGAGATCCATTACCGTTGAGTCTGCAG

3' Adjacent fDs: 226938620 (I.S07.4446_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|226938620 (135 bp)

>gi|226938620|gb|FI849942|I.S07.4446
CTTTTAAATGGTTAAAAACAACTGATAATTCTAGGTAAAGACGAATCGGAACCTAAGCATTTCGACAAGAATTGTGGTGGTCCAGTGCACGAGTCTCCGG
CCGTGGGAGGCACCGCTGTACGCGCGGTTCTGCAG



Genetics

Line was produced using a1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 2.7 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme PstI.
Predicted to produce a 2 kb band using primers LC24 LC18.
IPCR Notes:


Sequencing

IPCR product was processed by precipitation.
Sequencing reaction was performed using primers JSR05 and JSR03.
Sequencing Notes:

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