DS LOGO

I.S07.2829 (Ds Insertion Line)    View RefGen_v1 Placement     Admin

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diagram of ds insertion

Predicted Ds insertion site / closest B73 gene for I.S07.2829       Order Seed    View all Ds Insertions

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Loc
Predicted Ds insertion site   Closest Maize Gene
GDB Ds Distance from Gene All Ds
near
gene
Flanking sequence (fDs)
Quality
Score
Seq
Pos
1 3: 181,071,309 GRMZM2G108933_T01
[View Locus Details]
2.5 kb from 3` end gi|226938814 (I.S07.2829_JSR03) MQ2 5a
1 3: 181,072,074 GRMZM2G108933_T01
[View Locus Details]
3.3 kb from 3` end gi|226938594 (I.S07.2829_JSR05) MQ3 3a

Placement Details

More on Insertion Site...


This seedstock is available from the Ac/Ds Project

To order seed, use the online order form . You will need a purchase order from your institution to complete the order. When ordering seed, use the 9-11 digit barcode as the unique identifier ( I.S07.2829). Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order.

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Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 226938814 (I.S07.2829_JSR03) Blast@ZmGDB

Show FASTA Sequence for gi|226938814 (793 bp)

>gi|226938814|gb|FI849783|I.S07.2829
GAGAAGAAAAAGCAAAGCACGATGGCACCGCAGTACCGCACCGCTGCGTGCGCCCTGAAATTAAATGGGAGATGCTGCTGTGGCTGTGCACACGAATCTC
CTTTTTCTCCTCCTTCATGGACGCGACTGTGCGCGCGACGCCTGCATGCCCCCGACCCCCCAATAATTCACGTATAAAAAAAACTCACCAGTGGCTAGGA
TGACAAAGGCAAAAAAAGATGGAGCGTGATCGCAACAAGAGTACAGGACCTGGCCGTTCGTTTCCAGTGGCAGCTTTTAGATTTGCTCCGGCCGCCAAGT
CGTGTGTGTGTGCCTGCCGCTGCTGCTTCTAGGGTGCTATCGACATTAGTTTCTTATGCAGCATGATTGGGATATACTGATTATACTCTCTATTCTTCGA
TATGGGAGGGGAAAAAAAGAGGAAAGGGATATACATGGGATCGGGTCACGAACGGTCTCATGGAATCTGATGCTGCTGGCGTCAACCGGCCGAGCCCACG
GTATCTGACAAACAAATCAGACGTTCGTGAAAACGGCACGCATTGGGTCGATCTAGACATCTATGTCTAGCCGCGCGCGTGCTTTGAAAACGTTTCCAAG
CGCAGACAAAAAACCGGCACGTGGGCGCATGCAGGAAACAAACAAAACCGGAAACCTATCGCTGCCTCTGCTCGTCTTCTTCTCCACCAGATCTGCACCC
CCAAGGAAGAGCAGGAAAGAAGCATGGGGGCGCGCCACGCTCGATCGTATCCGGGGGAGGGGAAGAGGAAGCTTCCATGCAGCAGGATGCAGG

3' Adjacent fDs: 226938594 (I.S07.2829_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|226938594 (661 bp)

>gi|226938594|gb|FI849916|I.S07.2829
GCATGCAGGAACACGTTCACGACTCGCACTACGCTGGACGCTCTGCACTCGAGAGCGCGCATGCTCCCCCCCATACTCGCCTCGCCACTCAGAGCCCGCG
CGGCGATAGATAGTTCAGTGACGAAGCAAATAACAGGTGGCGCCCGCGCACTGCGCCTGCAATCTGGTGGTCGTGGTGGCGAGGTCGCAGGCAGCGATCT
GCTCGCACTTACTTTCGTTTGTAGTGTACTAGCTCGGTGGGAGCTGAAAAAAGCATGGGCAATTTGTGTGCGGCTGCCCATGGAGAGAGAGCGTCTCGGT
CGATCGTGCACTTTCGCACCAGTGGACGGTGCGTCACTGCTCCTCCTCTCCAGACTCCAGCCATACAGTATAACTGACTCAAACGGTTGCGTGCTGAGAC
GAGGCACTCACGCGATCCCTCGACCTCGCGAGGAGAGCTCACCACCGCCTGTCTGTTCGTGGGTAGCTCGACACGTTAGAGGGCAGGCGGGCGCCGCACT
GCGCTGCCGCTCGCCACCAGTTTGGCTGGCCTTGGGCTTCCCGTAGCAGCGTGCCGTCCACTTGCGCGGCGGAGGAGCCAAGAATCTCCAGACATGCCGC
TCTAAACAAGAGCAAGATGACAAATTCTGAATGGACGCGCGAGCTCCGTTTTCTCCTGCTG



Genetics

Line was produced using a1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 3.8 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme PstI.
Predicted to produce a 2.7 kb band using primers LC24 LC18.
IPCR Notes:


Sequencing

IPCR product was processed by precipitation.
Sequencing reaction was performed using primers JSR05 and JSR03.
Sequencing Notes:

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