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I.S06.2645 (Ds Insertion Line)    View RefGen_v1 Placement     Admin

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diagram of ds insertion

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Placement Details

More on Insertion Site...


Redundancy Group: RS.0029

More on redundancy...

This seedstock is available from the Maize Genetics Coop as AcDs-00210

To request seed of I.S06.2645 (AcDs-00210), you can add it to a Maize Coop Stock Center order -- this action takes you to the maizegdb.org website. Then complete the order on maizegdb, or use the "back" button to return to PlantGDB and additional searches. Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order (see below).

Before You Order...


Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 163955855 (I.S06.2645_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|163955855 (92 bp)

>gi|163955855|gb|ET189634|I.S06.2645
CCCACAGGCGCTTTCCATTTTCGTCTATCTCCCGAGAACCCTCCTGCGCCTCCCAAAGCTCTCTTGTTCGTTGCTCCCCCCCCCCCCCCCCC

3' Distal fDs: 163955856 (I.S06.2645_JSR04_JSR05_dir) Blast@ZmGDB

Show FASTA Sequence for gi|163955856 (181 bp)

>gi|163955856|gb|ET189635|I.S06.2645
CCCCCCCCCCCCCCCGTGCTGGTTAATCATCCCCTTCGATTTCGATCACATACATATAATCTCTCTCAGCTATCGCGGCCGCGCGCCTGATCGACGTACG
ACCGGTCTGGTACGTACGTGTGCTCGCCCGCCATGGCTCGCGGCGACGGCGAGCTGGAGCTGTACCGTGGGGGTACCGCGG



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 1.7 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme SacII.
Predicted to produce a 0.7 kb band using primers LC24 LC18.
IPCR Notes:

Product was used in PCR again using primers JGp2 LC45, producing a 0.6 kb band.


Sequencing

IPCR product was processed by precipitation.
Sequencing reaction was performed using primers JSR05 and JSR04.
Sequencing Notes: Coll. I.S06.1500

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