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I.S06.1602B (Ds Insertion Line)    View RefGen_v1 Placement     Admin

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diagram of ds insertion

Predicted Ds insertion site / closest B73 gene for I.S06.1602B       Order Seed    View all Ds Insertions

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Loc
Predicted Ds insertion site   Closest Maize Gene
GDB Ds Distance from Gene All Ds
near
gene
Flanking sequence (fDs)
Quality
Score
Seq
Pos
1 2: 17,868,249 GRMZM2G027891_T01
[View Locus Details]
-0.6 kb from 3` end gi|163955872 (I.S06.1602B_JSR05) HQ 3a

Placement Details

More on Insertion Site...


This seedstock is available from the Ac/Ds Project

To order seed, use the online order form . You will need a purchase order from your institution to complete the order. When ordering seed, use the 9-11 digit barcode as the unique identifier ( I.S06.1602B). Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order.

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Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 163955872 (I.S06.1602B_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|163955872 (695 bp)

>gi|163955872|gb|ET189651|I.S06.1602B
TACCTAATGGTACTAGTAATTATTTATTTAATTTATTTTAAAAATCCAGTTACCTTGACAACAAGAAGGAGCACTGGTACCCTGGCTCCATGGTGGTCCT
GCGCGACGTGGACGACACGAACAAGCTGAAGGCCTCCATCTGGTGTCGCCAGACGATCAACTTCCACGACATGGGGATGAGGTTCGAGCGGAGAGAACTG
CTGCTCCAGGAGATGATCAAGGTCCTGAGAGACCTCGAGATCGAGTACCGGATGCTGCCGCTCGACATCAACGTCCGGAACGCGCCTGCCATCCAGTCCT
CAAGGATGCCGTCGACATGGACGTTTAGTTATTGAGATGAGATTTTTGCTGCTAGAGGTAGTAGTTCCTGAGTGAGCTGAGCCGTGACAGCGTTCTGACG
AGAGGCGGCAACAGGTTTTGCTGTCGCTGCAGCCATCACTTGAGCTTTTCCTGTAGTTATTGTGTTGTTGGTCCAAGTACTTCATATAGTTATCTTTAGT
CCATCTCAGATATCTTTAGTTCATCTCAGTTATATTACCTGATGCTTGTCATTCTATTACAGATGGTATTGAATAATTGATTTCATCACTTGGGAATGAT
TTCTTCAGAATACATATGATGCTTGGGTGCGCCTGATGCTTGCCATTCTATTAACAGAAAGCTTGTGTGCAGCCCAATGCCTGATTAGTTGCTTT

3' Distal fDs: 163955873 (I.S06.1602B_JSR04_JSR05_dir) Blast@ZmGDB

Show FASTA Sequence for gi|163955873 (243 bp)

>gi|163955873|gb|ET189652|I.S06.1602B
TACGGTCGACATTTTTAAAATTGCGCAAGTTTTTTTTTTAAAGAGCAGGGAAGAATGCCTGGGGCGCTCTATAAAACTTGGGGGGTCGGAGGAGGAGGAC
GTAGGTATCGTGTCGGTGTGCAGTGTGTTGTCATCTCTAGCGCCGCCTTGCTCTGTGTGCTTCCGCCCGCGCTTGATCGCNTAACGTGAGCGTCGCTTCT
GCATTCCTGTGTTACTGTCGCCAGGGCTGCCTTGGCCCCGCGG



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 4.5 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme SacII.
Predicted to produce a 3.5 kb band using primers LC24 LC18.
IPCR Notes:

Product was used in PCR again using primers JGp2 LC45, producing a 3.2 kb band.


Sequencing

IPCR product was processed by gel extraction.
Sequencing reaction was performed using primers JSR05 and JSR04.
Sequencing Notes: Two Reads

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