DS LOGO

I.S06.0422 (Ds Insertion Line)    View RefGen_v1 Placement     Admin

Jump to Record:



diagram of ds insertion

Predicted Ds insertion site / closest B73 gene for I.S06.0422       Order Seed    View all Ds Insertions

Alt
Loc
Predicted Ds insertion site   Closest Maize Gene
GDB Ds Distance from Gene All Ds
near
gene
Flanking sequence (fDs)
Quality
Score
Seq
Pos
1 5: 179,008,969 GRMZM2G013324_T01
[View Locus Details]
1.4 kb from 5` end gi|170762509 (I.S06.0422_JSR03) MQ3 5a
1 5: 179,008,960 GRMZM2G013324_T01
[View Locus Details]
1.4 kb from 5` end gi|154359654 (I.S06.0422_JSR05) MQ2 3a
1 5: 179,008,960 GRMZM2G013324_T01
[View Locus Details]
1.4 kb from 5` end gi|154359655 (I.S06.0422_JSR04_JSR05_dir) MQ3 3d

Placement Details

More on Insertion Site...


This seedstock is available from the Maize Genetics Coop as AcDs-00482

To request seed of I.S06.0422 (AcDs-00482), you can add it to a Maize Coop Stock Center order -- this action takes you to the maizegdb.org website. Then complete the order on maizegdb, or use the "back" button to return to PlantGDB and additional searches. Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order (see below).

Before You Order...


Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 170762509 (I.S06.0422_JSR03) Blast@ZmGDB

Show FASTA Sequence for gi|170762509 (628 bp)

>gi|170762509|gb|ET629031|I.S06.0422
CCTGGCGGTGCTAGTGTAGCGGTAGCCGTGTCCGTGGGCGTGGTGGAGAGGAGAGGGGAGGGGCGGGTGCAGCACCAAGGAAAAGCATGAGATGCTAGCG
GTGCTGCTCGGCGTGCTTGCACGGGAGCCGGGAGCCCTGCCTGGCTGTGGCTGCCTGTGCTCATCGTTGTTGCTTGACACAGCCACATAGGGCACCGTGT
CTACTCGCTCGCCCACCGACGGTTTAACTAATCACGGTGCCTAGATTTTAACTAATAAATAATTTGACTGTGTGCTACCTCGGAACCCGGTCGTCCAAGT
TACTGTAGTAGCTGGAGTACTACTACTACTACTAGTGGCCCCCCGCGACAGGAGAAATGCGCATCGTGTCCGTGTGCCCCCTGCCCTACCACTACAAGAG
GGTAGGTAGGATAGGAAGGTAAGCCAGCAACTTCCCGTTCTTTCCTAGCTAGTAGCGCGACAGGCTATCGGGTTACAAATGTACTGCGTCGGCCTTTCGG
GTAATTTTGCCACATGACCAGACCTTCTGTTTCTGTGAGGGCAGGCATATCAGTTCAGTGCGATCAAACAGCTTATTTCCAGACGTGTTTTGGTTACGAT
GGTGGATATCCATAATGTCTTTTTTTTT

3' Adjacent fDs: 154359654 (I.S06.0422_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|154359654 (666 bp)

>gi|154359654|gb|ER973911|I.S06.0422
CCGCCAGGCGGCCAGCCAGCCAGCCACAGCGCACCGCGCAGCCCAGCCTATGATGAGCTATCATGATTACGATCCAGTCCAAAAAAACCACATGCACTCT
TTTATTGCCCTCGTACAATCACCATACAGAGATGTGGAAAAGGGGGTCCCTCTATCTCCCCTCGATCAAGCCGGCCTCGCATCATTTCGGAGCTGTTTGT
TTTTACTGGTGGTTTGCCTTTACTCCATCCGTGCAGGGGGTGGAAGGTGTCGTTTTTTTCTTCAAGTCAAGACAATATATTTTCAGTTAAATATGATAAA
TATATTAATATTTATGATGTGCAATAATCACTACATTAACTGTAGAACATAGTAGTATATCTTTACGATAATCTAATGGAAGATCCGGATTTAGGCAATA
TCTTTAATCGAATTAATAAAGATTGTTTAGCTTCCAGAGACAGCTTTCGTTGTGGGTTTTTTATTTGGTGGAGGCAGTGGTACGCTAGAGCATACAAACA
TCTTTGGAGTTTTTTAAACTAAAAATATCACTTGTTTGGCAAAACATTAGCCTCTACGAACTTATGGACATACTCGTCTCGGGCTCAGTGAACTCTTTTT
TTTTAAAAAAAATTCTATTGTTACAAATTAATACGACACAAAGAGGATCAATCAGAAAGACACGGA

3' Distal fDs: 154359655 (I.S06.0422_JSR04_JSR05_dir) Blast@ZmGDB

Show FASTA Sequence for gi|154359655 (551 bp)

>gi|154359655|gb|ER973912|I.S06.0422
AAATCAAAAATGCAGTTAAGACTAGCGGCGAATCGAAGATTTCGTAAAGAGCCTGGACTACTATATGAAGAACTAACAAACACCCTCACGTTTTTTCGGC
TCGTGCACATCACAAAGTCATCGAGAAAACGTCACTGGAAGTAATAAAATCAAAACAATAGAGAGAGTGAACATTTTCACCGAAGAAAAGGAAAAACGAA
TGGAACTAATTATTTCTTTTTTTTCTCTCCTTGGAAAAGTTTGGAAATTAGACCAATTCGGAGCATCTCTCTGACCACCTTCCCCTCCCCCTGAGGCCCT
GACCACCTTCTCCTCCTGTACACAGCTCAGCACTTCAGCAGCAGCATCGCATCATATCGCCCTCGTCTCCAGTTCTCCTCTCTCTCGGTCTTAAAAATCC
AGCGCCAACGCGGCCGCCGGACGCGCATTGCTCTCCCTCCCTGCCTCGTCTCCTCACCTCATTCTCGCCACATCTTCCTCTGCTCCCCTCCTCCCAGGTG
GCGTTCTTGGAGCTCCAGCTCCTCGAGCTGCGTCTGTCTACGCCGCCGCGG



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 1.9 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme PvuII.
Predicted to produce a 0.9 kb band using primers LC24 LC18.
IPCR Notes:

Product was used in PCR again using primers JSR01 LC18, producing a 0.75 kb band.


Sequencing

IPCR product was processed by precipitation.
Sequencing reaction was performed using primers JSR03n and LC18.
Sequencing Notes: none

Loading Help Page...Thanks for your patience!

Loading Video...Thanks for your patience!

Loading Image...Thanks for your patience!