DS LOGO

B.S07.1312 (Ds Insertion Line)    View RefGen_v1 Placement     Admin

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diagram of ds insertion

Predicted Ds insertion site / closest B73 gene for B.S07.1312       Order Seed    View all Ds Insertions

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Loc
Predicted Ds insertion site   Closest Maize Gene
GDB Ds Distance from Gene All Ds
near
gene
Flanking sequence (fDs)
Quality
Score
Seq
Pos
1 9: 138,754,659 GRMZM2G162486_T01
[View Locus Details]
-0.1 kb from 3` end gi|226938646 (B.S07.1312_JSR05) MQ2 3a
1 9: 138,754,659 GRMZM2G162486_T01
[View Locus Details]
-0.1 kb from 3` end gi|226938647 (B.S07.1312_JSR04_JSR05_dir) MQ2 3d

Placement Details

More on Insertion Site...


This seedstock is available from the Maize Genetics Coop as AcDs-00295

To request seed of B.S07.1312 (AcDs-00295), you can add it to a Maize Coop Stock Center order -- this action takes you to the maizegdb.org website. Then complete the order on maizegdb, or use the "back" button to return to PlantGDB and additional searches. Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order (see below).

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Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 226938646 (B.S07.1312_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|226938646 (968 bp)

>gi|226938646|gb|FI849635|B.S07.1312
CAGAACACACTGCACATCATCATTCTTTCCCACCCATTCATATATATACAGTGACATCGACACGTCACCAAAGCAACGATAATACCTGGACAACTAGTTT
ATTTATTTTCAGAAGCACCGCCATTACAGAAGGCCATGCCTTATTGAAGACCAGATGTAATATCCAAGCACACAACCGACTAAAACGAGCTCCGATCGAT
CTACTACGCTGGTGCTTCTGGCGAAAGTCTCGTCCTTGGTCACAGTCTTTAAGCAATCTGGATGTTCAAAACGAAGGTTGGTCAAATGTTTTGAAATTAG
CTGAACAAATATAGATCGATCGATCAAGGCAGAACAAAATTAAGAAATACGGAGTGTTCAATACCCCAAGCCGCTTCTTTGTTTGCTCAAGCAAGAACTG
CGGGTCCAGTTTAGTCTGTGTGTACGCATCGATCTTGTTTACTTCCTGAGACATACCGACCCGTGAGTTAAAGTTTCTCATCAAGGAAGCATTGTCAACA
GGATAAAAGGATCGTCTCAAGCAATGTTAGTGAACGAACGTATTACAGATATAAATCCTAGTCAATTTGAAATCCATCGGCGTGAGCTACCTCGATGAAT
TTCTGAAGGTTGGGTCTGCCCTTTGTGACGTCATAGTTCTTTATATTGGAATAGAGTATCTGAAACCTCTCGATGAACGGTACATACGCAACGTCCACCT
GTTCGTGTAATAATGAAAAAAACGCATCACGACAAAACCACATGCCAACTGAAGCCCACATTCGTAATGTAGATTTGATTTTGAACCCTGCTCCCGGGAC
GGGAGAGAACGATTCCTAGTGTTCAGCGACACATACCAGACTGAACTGGCCAAGGAAGAACGGGCCGTCGTTGAATTTCCCCAGGGCGTCTTCTATTTTG
TCCAGGCAGCAACTGATGTAAGACTGGAATGAGGCATTAGCATCTTATTGAAAGACTTTTTTTCCAGA

3' Distal fDs: 226938647 (B.S07.1312_JSR04_JSR05_dir) Blast@ZmGDB

Show FASTA Sequence for gi|226938647 (741 bp)

>gi|226938647|gb|FI849636|B.S07.1312
TTTTCTGGATAAACCTTCTCCTTGTACCACGCTGGCCTGTCAGCGAGATCGATGGCAACTATCTTAATCTTTTCCTGCAGACCCTGGACAGACGGCGAGG
AACAGAAAAGATTTCAGTTTTCCTTTCCCGGAAGACACCACAAATTGAGGAGAACCGCTTCAACAAATCAGAGAACGGTGGTGATGGTGATGGTACCACC
TTGTAGTTTCTAGCAATCCAAGCGCGCTGCGCGAACGGGCAAAGATAGGCCACGTACAACCTTCACGAGACTCAAAAACCGAACCGGGGGTTAACTTCTA
GGTCATGGTCTGCACAGGTGAAGGTTTGTAGTAACTAATTGATTGATAGGTCGAAAACCTACCTGGTGGTGCCATCGAAAAGGGGAGGGGGCTCGGAGGC
GGAGGTCAGGGGAGACGGCAGCACTTCCTTCACGGAGCTGCACAGGAAGGAGGAAGGACTGTTCCATTAAAAAACTAGCTAAAGAATCTCCCTCCACAGA
AAGTTCAACAAACAATCGTTTTTATACAGGGGGGGGAGTTAGATCAAGAATCTGAACGGCGACAGCAGACAGAGACAGACAGGACGGCGTTCTGCTCAGT
GCTCACCTTGCAGGAGCGGCGGCTGCCATGGCGGCGATGCGGCCGGTTCGGGAGGAGCTTCGAGCGTGGAGGTGGCGGTGAGCTGCATCTTCTCGTTGCC
GGCAGCGAACCTTTATCCGACGGCGATGGGAAGGGCCGCGG



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 4 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme SacII.
Predicted to produce a 3 kb band using primers LC24 LC18.
IPCR Notes:

Product was used in PCR again using primers JGp2 LC45, producing a 2.8 kb band.


Sequencing

IPCR product was processed by precipitation.
Sequencing reaction was performed using primers JSR05 and JSR04.
Sequencing Notes: failed sequencing

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