5', 3', Adjacent, Distal
Quality refers to the Megablast % identity/coverage score:
Alt refers to the alternate locations under DP:
To request seed of B.S06.0142 (AcDs-00020), you can add it to a Maize Coop Stock Center order -- this action takes you to the maizegdb.org website. Then complete the order on maizegdb, or use the "back" button to return to PlantGDB and additional searches. Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order (see below).
Please verify that this is indeed the correct seedstock for your needs. Click under Placement Details section above for more information about potential Ds chromosomal location(s), based on fDs matches to genomic DNA. Please note that multiple, ambiguous placement locations are possible. You can run your own blastn analysis for any fDs using this link: Blast@ZmGDB. See AcDs Help for more information on insertion sites.
Other data related to this seedstock may be present: Redundancy Group --where multiple barcodes map to the same region; and Confirmation-- if location has been confirmed by molecular methods. Other sections below give details on fDs Sequence, Genetics, PCR conditions and Sequencing Methods. Please contact us if you have any questions.
W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:
3' Adjacent fDs: 148608146 (B.S06.0142_JSR05) Blast@ZmGDB
3' Distal fDs: 148608147 (B.S06.0142_JSR04_JSR05_dir) Blast@ZmGDB
Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 3.4 kb.Southern Notes:
Genomic DNA digestion performed using the restriction enzyme SacII.
Predicted to produce a 2.4 kb band using primers LC24 LC18.
Product was used in PCR again using primers JGp2 LC45, producing a 2 kb band.
IPCR product was processed by ethanol precipitation.
Sequencing reaction was performed using primers JSR05 and JSR04.
Sequencing Notes: Two Reads
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