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B.S05.0699 (Ds Insertion Line)    View RefGen_v1 Placement     Admin

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diagram of ds insertion

Predicted Ds insertion site / closest B73 gene for B.S05.0699       Order Seed    View all Ds Insertions

Alt
Loc
Predicted Ds insertion site   Closest Maize Gene
GDB Ds Distance from Gene All Ds
near
gene
Flanking sequence (fDs)
Quality
Score
Seq
Pos
1 2: 197,221,263 GRMZM2G049613_T01
[View Locus Details]
0.7 kb from 5` end gi|133823237 (B.S05.0699_JSR05) MQ1 3a
1 2: 197,216,866 GRMZM2G049613_T01
[View Locus Details]
5.1 kb from 5` end gi|133823239 (B.S05.0699_JSR04_JSR05_dir) MQ2 3d

Placement Details

More on Insertion Site...


This seedstock is available from the Ac/Ds Project

To order seed, use the online order form . You will need a purchase order from your institution to complete the order. When ordering seed, use the 9-11 digit barcode as the unique identifier ( B.S05.0699). Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order.

Before You Order...


Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 133823237 (B.S05.0699_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|133823237 (452 bp)

>gi|133823237|gb|EI414177|B.S05.0699
GTGGAGGGGCTGGCTGGATTTTACCCTGCTGCGCTGCCGCTGCCGCTGCCAGCCCACCGGCCACCACACACTTGTTGGGGTGCGGTTTTTTCCGCTTCGT
TTCGTTTCCCCCCTCTTTATTTCTCTCGCTTTCTTCCTTGGCTTATTTATTATTACTTCCTCCTCTCTTTTCTGATCTGAGTCATGCGTCTTGCTCTCTG
CTCCCCGTTCTTTGTCACGTACTTGCTGCTGTTTACTAACAACGATGGTGGCTGCTGGTGACCTGGAAGTTTGTTGTGGGGCGGTGGAGAGGGCGTGGCT
GACGAGAGATGGTACGAATTGGTGTGTGTGGTTTCGTGTGAGTGATCAGCTGATCATCCATCAGACTGTGTCTGGTGTGAAGGTTTTTTTTAATGAATCC
GGGTACTGCCAGCCGCTGCTGGATTTTTTTTTTATCCGGGGGTTTCTGCTTG

3' Distal fDs: 133823239 (B.S05.0699_JSR04_JSR05_dir) Blast@ZmGDB

Show FASTA Sequence for gi|133823239 (224 bp)

>gi|133823239|gb|EI414178|B.S05.0699
TGTGCGATAACGACTAGTTGAAAAACTTGATCTTAATAAACTCCAAGAGACACGATATATGATTTTGTAAAGCTAAATTTATCTATTATGAGAACAAAAA
ACGTTTCAATAAATGGCTCTATTAAAAATTTAATAGGTCTTCATCTTTTCAAACGTGTGGTAAACCTTCCTTTGGTTGCTCTGCTCTCGTTGCTGCGGCA
TCCGCCGCTTCCTCTACCCCGCGG



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 3.8 kb.
Southern Notes:

View Image: Southern Blot showing B.S05.0699
(Click image for larger version)

Southern Image

Inverse PCR

Genomic DNA digestion performed using the restriction enzyme SacII.
Predicted to produce a 2.8 kb band using primers LC24 LC18.
IPCR Notes:

Product was used in PCR again using primers JGp2 LC45, producing a 2.8 kb band.


Sequencing

IPCR product was processed by gel purified-freeze dried.
Sequencing reaction was performed using primers JSR05 and JSR04.
Sequencing Notes: Two Reads

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