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B.S05.0073 (Ds Insertion Line)    View RefGen_v1 Placement     Admin

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diagram of ds insertion

Predicted Ds insertion site / closest B73 gene for B.S05.0073       Order Seed    View all Ds Insertions

Alt
Loc
Predicted Ds insertion site   Closest Maize Gene
GDB Ds Distance from Gene All Ds
near
gene
Flanking sequence (fDs)
Quality
Score
Seq
Pos
1 5: 6,276,372 GRMZM2G034877_T01
[View Locus Details]
3.6 kb from 5` end gi|140230937 (B.S05.0073_JSR05) HQ 3a
1 5: 6,276,372 GRMZM2G034877_T01
[View Locus Details]
3.6 kb from 5` end gi|140230938 (B.S05.0073_JSR04_JSR05_dir) MQ3 3d

Placement Details

More on Insertion Site...


This seedstock is available from the Maize Genetics Coop as AcDs-00514

To request seed of B.S05.0073 (AcDs-00514), you can add it to a Maize Coop Stock Center order -- this action takes you to the maizegdb.org website. Then complete the order on maizegdb, or use the "back" button to return to PlantGDB and additional searches. Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order (see below).

Before You Order...


Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 140230937 (B.S05.0073_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|140230937 (726 bp)

>gi|140230937|gb|EI505364|B.S05.0073
ATTAGCCCTCCATGGTGGGCTCCTTTTGTAATTGAATTTTCTTCTTTATGTACAGAACAACACCCGGAGAAAACAATTGTGTTGTTAATCATTAATCTTA
CACAAAACGACCCCACCTATATTTTCAAGACAAACAATAGGAAATTGTACTTATTATAGTTTTGGGAATATATTTTTTATTCATTGACAGCGCTAGTGCC
AAGTTACTCTCATAATTTCTTTGCGAGAAAGTTACTCATATAAATGATAAATATAAAATAGAACAACATCCAACTGAGCTTCTCAGGTACTTCAGTTTGG
ATTAGTAGGAACTTGGAAGAACTGTTTACCATGCTTTAGAATAGCCATATCTTTCTTCAATTTAGATATCAACATGTTTTTCCTTTTAAGAACTTCTTGC
AGCTCTGCAGTTCTCTACATGCCAGAAAGGAAGATTTTACCAATACTCCAATAGTATATTACCTGAAGTGTTAGTAGTGACAAATAATGAGATTCTACAA
GACAACTGAAAAGATGGTAGATCAACCTCTAGATCAGGTAAAATGGCTAGCTTCTAACAGATCAAAATGACTAATCAAACTACCACATCTCAGAACAGAG
GGAGAAACCATACTAGAATTAATATGAAAACATGATATAGAATCCATCGGGGATGAACCCAAGACAAAATGAGAGTGTGGGCATTCCTTGTAAGGATGAG
CATCCAAATGATAGAGTAGGGGCCTC

3' Distal fDs: 140230938 (B.S05.0073_JSR04_JSR05_dir) Blast@ZmGDB

Show FASTA Sequence for gi|140230938 (612 bp)

>gi|140230938|gb|EI505365|B.S05.0073
AAATAAATTTTAGCACCAATCAACATGTCACAGATAAAAAATTGAAAATTAGAAATTAGGTTAATATATTAAATCTGGATGGCCCACATGTCAGATATCA
ATTAGGTAATAGGTATGAATACTGAATATCCTTACTCGCAAAAAAATTATCCATAGATATATTATTATTCATAATCATACTCACATATATCAAATTCTAT
CATAACTATATTCAGTAAATAATTATTGATAGTTATTTATCCATACCCGTGTCCACTGTGATCCCGCCGATAGCCTCCAAGACGGAGCTGGTCTCCCAGA
AAAACAACTAAGAACTAACCAACCCCGACTCCCGCCCGAGACACCAGTGAGAAAAAGACAAAACGGCAGGGGGTCTTCCCTCCCTCCGCGACACCATCTC
CGCCACCTCATGATCACTAACGTTTCCCTCTTCTACCCATTAGCGACTTCCCGATGTGCTGGTGGAAGCGCTACTCCTCCCCCTCCCTCTCCTCATCATC
CATGCCCGCTTCCCCCGCCCGCGCTTCCATCCCCCGTTCCATATTCGGTTGGGGCAGCGGCCCTAGCTCCCGCCGTGATGGCAGCGGGGGCAGCAGCGAA
CCGCAGCCGCGG



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 5 kb.
Southern Notes:

View Image: Southern Blot showing B.S05.0073
(Click image for larger version)

Southern Image

Inverse PCR

Genomic DNA digestion performed using the restriction enzyme SacII.
Predicted to produce a 4 kb band using primers LC24 LC18.
IPCR Notes:

Product was used in PCR again using primers JGp2 LC45, producing a 4 kb band.


Sequencing

IPCR product was processed by gel purified-freeze dried.
Sequencing reaction was performed using primers JSR05 and JSR04.
Sequencing Notes: Two Reads

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