DS LOGO

Ac.mon03068 (Ds Insertion Line)    View RefGen_v1 Placement     Admin

Jump to Record:



diagram of ds insertion

Predicted Ds insertion site / closest B73 gene for Ac.mon03068       Order Seed    View all Ds Insertions

Alt
Loc
Predicted Ds insertion site   Closest Maize Gene
GDB Ds Distance from Gene All Ds
near
gene
Flanking sequence (fDs)
Quality
Score
Seq
Pos
1 7: 120,894,805 GRMZM2G147966_T01
[View Locus Details]
116 kb from 5` end gi|70673332 (Ac.mon03068_JSR05) MQ3 3a

Placement Details

More on Insertion Site...


This seedstock is available from the Ac/Ds Project

To order seed, use the online order form . You will need a purchase order from your institution to complete the order. When ordering seed, use the 9-11 digit barcode as the unique identifier ( Ac.mon03068). Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order.

Before You Order...


Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 70673332 (Ac.mon03068_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|70673332 (1294 bp)

>gi|70673332|gb|AY618479|Ac.mon03068
TCAGCAGCAACGACCAAAAACTCCAAGCATCGCCTGGTGCAGCAGCTGCACACATCACGGCAAGGCTATCTATCAGATGGAGTAGGTAAAATAACTAAAT
CTGTCGCCCTCCACTTCACTCAGTAGGCGCAAGATTAGGARGGAAGCTTTGGGTGAAATGACGCATGGCTTATGATTTGTAAATGATTGTGTCAGGTGTG
CGCGGTTATTCTCGAGACCTTAGCATGTTTTTATTTTATTTTTTATCAATTGCATTGCAAAAAAAAAAAAATTTTGAAATCTTTAACGCGTTGGTCTTTT
CGGAACCATGCGAAAACTAGAGGTGGTCGCCAGGYGAAAACAGGTAGGTCGGGTATATCCGCCGAATACAGATAATATAAATACATATTTTCTTAGAGTT
AGATACGAATAATATAAATACCTAATGGTCGCCGTGTCATCGCAGTCCATAGCCGGCGGCCTTGGAAGTCCTGCGTATTGACATCTTTGCTGCTGGTTAT
TTAAAGTATCTTTTGCAATCCGTTACCTACCCCATGGAGTCATGAAACAGTACATAATTATATGACTAACTGTACGCCCAGTTCCTTCCATTTTCTTTTC
TTTTTTCCCGTTGAGCATGCCTAGTTATTTCCACTAGAAATTGATGGAAAACTGGAGGACCGAGGACAGCGAGCAAATACTGGGTGGAAGTGGAACCGAG
GCCGCTGGTCGCTGGATCGCAACGTCAGCCGTGGTTGGTAAACGTTGAGGTCATGACGGCAACACATAGTTTCCATCGTCTCAATGCAGCTGCCCGATCC
TCCTCCATCTGACACTGATACTTCCAAGTTGTGTAGTATATTTTTGCATCGTACCGTACCATGACCATACCACCTTGATTGCCGTTGTAACTAATGCGTC
CTTGAAGGATGCTGATGGATTAGTCCGTGAGACTGTACGTAGCAGATCATGTAAAATAGGGATTTGTTCTCTAAATTACAGGATTTATAGAGTGAAAATT
GAAATAGTGGATGTGATAAAGGAATRACATAGATACATGTTGAAGACAGTCTGACACTGCTTCTGCCAACGGTGGTGGGCCCATGCGGTCGCAAAAAAGA
CGAATGCAGCACGTCGATTAACCTTGATTGCAGCACGTCGATGCCATGGACCAGTCACCATTGCCACCGGGATCTACCTGCTTGACAACACGCGCGCCAC
AGGTGGGCAGATCGTCGATTAACCTATGCTTCTCAGGTCTAGCCGAGGGTAGAAATTCGTAGTAGCTTACAAATCACCTGCTTGGACTTTGCTC



Genetics

Line was produced using as a donor.
Ds element donor under selection for transpositions.
Identified as Southern band of kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme .
Predicted to produce a kb band using primers .
IPCR Notes:


Sequencing

IPCR product was processed by .
Sequencing reaction was performed using primers JSR05 and .
Sequencing Notes:

Loading Help Page...Thanks for your patience!

Loading Video...Thanks for your patience!

Loading Image...Thanks for your patience!