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Ac.bti00191 (Ds Insertion Line) View RefGen_v1 Placement Admin

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Predicted Ds insertion site / closest B73 gene for Ac.bti00191 Order Seed View all Ds Insertions

Alt Loc	Predicted Ds insertion site	Closest Maize Gene	GDB	Ds Distance from Gene	All Ds near gene	Flanking sequence (fDs)	Quality Score	Seq Pos
1	2: 3,597,862	GRMZM2G365162_T01 [View Locus Details]		-0.1 kb from 5` end		gi\|54292167 (Ac.bti00191_JSR05)	MQ3	3a

Placement Details

Insertion site details for Ac.bti00191

	fDs Placement				fDs Placement alternative (if any)
fDs sequence position -⟩
Sequence Type -⟩	5' Distal	5' Adjacent	3' Adjacent	3' Distal	5' Distal	5' Adjacent	3' Adjacent	3' Distal
fDs CloneID			Ac.bti00191_JSR05
fDs gi			54292167
fDs Acc			AY559204
fDs Seq Length			1434
Single/Dual Placement			SP
Placement Quality			MQ3
Likely Ds Insertion Site Click to view at ZmGDB			2: 3,597,862
Closest Gene (Click to view) Ds Distance (red=inside gene)			GRMZM2G365162_T01 (-0.1 kb from 5' end)
Gene BLASTp top hit Click to view UniRef record			filtered_set; syntelog; UniRef90: Q7XM23_ORYSJ Os04g0662200 protein n=3 (Poaceae) Exp=4e-45; maizesequence.org: UniGene:Zm.124003; TSA: Zea mays conti...
BLAST fDs @ZmGDB (Zm-genome masked)			Blast@ZmGDB
Chr			2
fDs Left Coord			3,595,677
fDs Right Coord			3,597,861
fDs Orientation			-

Click for explanation of location, placement (SP, DP) and quality (HQ, MQ1, etc.)

This seedstock is available from the Maize Genetics Coop as T3301-01

To request seed of Ac.bti00191 (T3301-01), you can add it to a Maize Coop Stock Center order -- this action takes you to the maizegdb.org website. Then complete the order on maizegdb, or use the "back" button to return to PlantGDB and additional searches. Please note that genomic location reported here is subject to error, so we advise you do careful research before placing your order (see below).

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Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 54292167 (Ac.bti00191_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|54292167 (1434 bp)

>gi|54292167|gb|AY559204|Ac.bti00191
GAGAGGTGGCTTGGACTTGGAGGCAGACTTGCGGAGGGGAAGGTAGCTAGGCTTTATAGGGAGGAGGGGCCCCTYGGCGACCCTTTGCCTTTCGGATCGT
CCAGTTGGCCCGGTATAAAATTATGCTGAAAAGGATGGATTGTTATTCTTCTATTCTTCTATTCTACTACTCTCTGTAATTTGACATTGTCTATGTCTTT
TTTTTTTTTGAGAGAGAGAGGGGTTGCAGGATGGGCGTGAAGAACTGGGCAATGGAGACAAGAACTCACAAGATGGTTATACAAAACTACATCCTGTGTT
TATAGAACTATTAGAGAAATTACAGTTTGTTACTCTCAAACTTATTTAGCRACTTGTTAAATCTACGTAGAGAGCTAAAATTTACATAACTATTGGAGTT
GCTCTTAGAGTTGCGCTTAACCATGTCGTGACTCTCATTCAGAGTGGAACTGCACACATGTATAAACGGAGGAAATAAAAGTCCATCCATCTAACAAAAG
TATTTTTTTCCCTGAACTATTATTGGTAAGCGCACATGCTTAACGTTTTAAAACCGCACCGTCAAATGCCTGCCTGCCTGCCTAAACTATGCAGCTTGCA
CACCCTTGTGCTACCAAGAAATGGAGCGCACAAACTTCCCTGCCTCTTGCGCATCCGCAGATCAGCAGATGCCATTTTCGCCGCTGCACTTGTTCAACTC
GCTCCCCTCAAGTTGTGCATGCACGGGTGATAGGTGCACCGTGCAGGGTACACCACGCTGCTCGTGTTCTTCCTCAAACTCGGAATACGAGCGCAGTGGC
GGGGACAAGTTTTTGTCACTTTGCAAACGTGCGGAACCAAACGAAGCTCGCTGTTGCAGTGTTTTCTTCGTGTCGCGTCGGACAGTGAACAGAGCAGACC
GGAGTAGGTAGACAGTGAAGAGAGCACTGCACAACATTGTGTCCGTAGCATCGCATCGTGGCCGGGGCCAGCAGTCGCGTCACACCATCGGCGGCGGTGT
CGAGAGGCCAGGCGAGGTGCCCCCCTCTCTCYGATCTCTCCGAACTCTGACAGGTGAGGTGAGCGAGACGAGTGGCGRCCCAGRGTGTGGGAAGTGCGCT
GCTGCTGGTGCATGTGACCGCAGCCGGGCGCGCATGTGCACGAACCAGTGGCCTGTCTGGTCCGGCCGATCTCTCCTCGGATTTTTGCGCGCAGTTCTCT
GCCGGGGCCCGTGGCTTTCGAGGATCGCCGGGAAGATTTCGCGGCACGCCCAGCAGCATCCAGCAAAACTTGCCGGTAACCCCGTGTGTCCTGGTGTGGT
GGCTATGCAACGGAGCTAGGTGCGGTGCAGCAGAGAGAGAGGCTTCGTACTAGCATGTTTCCCTGGCTGAACACTTTTTGTCAAACTCTCTTTCCGCGTG
GAGTAGGAGTAGCGTCCAAGCTTGCAAAACTGCA

Genetics

Line was produced using as a donor.
Ds element donor under selection for transpositions.
Identified as Southern band of kb.
Southern Notes:

Inverse PCR

Genomic DNA digestion performed using the restriction enzyme .
Predicted to produce a kb band using primers .
IPCR Notes:

Sequencing

IPCR product was processed by .
Sequencing reaction was performed using primers JSR05 and .
Sequencing Notes: