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I.S07.4196 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 226938577 (I.S07.4196_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|226938577 (150 bp)

>gi|226938577|gb|FI849899|I.S07.4196
GTTCAGGGCAACACTCTTGCCACTTTGCTTGCTTAGAAATATTCTCTATCCTTTATAGTAGTCTCCAAAAGATTTTATGATGATGTCGAATTGTGTTGCC
CTCATCCTTTTCTGTCTAACATCTACTGCAATCATGCATAAGGTTCAGGG



Genetics

Line was produced using as a donor.
Ds element donor under selection for transpositions.
Identified as Southern band of 0 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme .
Predicted to produce a 0 kb band using primers .
IPCR Notes:


Sequencing

IPCR product was processed by .
Sequencing reaction was performed using primers and .
Sequencing Notes:

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