DS LOGO

I.S07.1236 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Ds location and closest gene       Order Seed    View all Ds Insertions

Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr1:13,586,941 GRMZM2G004641_T01 1.9 kb from 5` end gi|198400533 (I.S07.1236_JSR05) 3`Adj
1 Chr1:13,616,375 GRMZM2G417455_T02 6.7 kb from 3` end gi|198400534 (I.S07.1236_JSR04_JSR05_dir) 3`Dist
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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 198400533 (I.S07.1236_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|198400533 (791 bp)

>gi|198400533|gb|FI187585|I.S07.1236
GGCCGGGGGTATCCGTTCAGCTGTATTAGTCTGGTAACCACCAGTTGGTGGACCTAAACCCCGCTTTGCCCTGTGAACAACTTCCGCCACCACAACTGAT
CCGACGACCATCATCATCATCATCATTAGATGCCGGCCTCGATCAGAGCTAGAGACGATCGACGCAGGACCAGTAGCAAGTGCATCGGCAACAGAAACGT
GCCACCAGCATGCAGCAGCAGATGCGTAGTACGTACGTTCGTACGTGTAATGCTGCGCCTGCACTGCACGCCCAAAGGCGGCCAAATAATCTCCGCTCTA
TCTTTTTCGTTTCGTTTCTGGTTAAAAGTGCAGAGGGTGTGCCGGCCCCCTAACACCACTTAGACACTAATGCCCGCCTTTTATCTCGATCATCGGTATG
CATGGTGGCAGAGCACTTGCTTTTGATAGTATGATGGGATCGACATGTGGTGGTGTGGTAGTGATGCATGCGCAAGGGACACGAGTGTCCCGTTTATTGG
TCCCCCCCTCTCTCCAACAATGAACTTTGACCCTTCCTCCAACAATTAACAACACCTTCCATTGATCTTCTCTCTCGGCAGAGTCTCCAGCACACGGGAC
GGGATCTATTGCCGGCCACATGTCTTTGTTACTCAGATCAGAATCTGATAGGTGTTGAGTAGTGTATGCGTTCCTCAAGATCGCGAATGTACGTTCATGG
GTTTCAGGCAGCGCGAGCGAATGCGACGCGCCCATGCCAGCCTGGGAAAATCCGAAATTTTCCTCAAGATTGGATGTTTTTTCACTTTTTT

3' Distal fDs: 198400534 (I.S07.1236_JSR04_JSR05_dir) Blast@ZmGDB

Show FASTA Sequence for gi|198400534 (739 bp)

>gi|198400534|gb|FI187586|I.S07.1236
CTTTTTTTTTGTTGTAGAGAGAAAATAATGTACATAAAAAAGTATACAAAAAATTATTTAAATACTTCATAATATTCTTCATAACATTGCTAAAAATTAG
CTTTAAATCATATCTATATCTATGCTAAAATATTAGATTTGTGATACAAGTCGGATGCATCCCTACCAGTGTCCAGTTGCCTGGTGTGATTCACGAACGA
CACGCTATAAGAAAAAGAAAATAATAATGTGTGGGTATCGTCGTAGTCGTCCGGCACTGAGCTGAAGACGGAGAGGTTGAGATGCTCCACGCCGCCTAGG
TAGAAATGTGAAGGTCACGTGCCTTGTTTCTGAAGAAACAAACAAAGCACGCACGCGCGCGCGCCCTAACAAGATTTAGTTACAGGCGTCCCATCAGACT
GAACGGAGGAACCTGACCAGCTACGGCGCATGCATCTGGTTCGGATGGATGGATGACCCTGTGCCTCCTCCTATTGCCACCCGACGCCATTGTCGGATCC
TGACGTCTCTCTCTCACTCACTCTCTGCTCGCTGTCTTTTTCCGGGAAGAAGGCGCCGGTGCTGGCCTGGCCCCCCGGCGGCAGAGAAGTTTCTGATCAT
GGCCCGCGCGCGCCTTCGTGGGAAGGGATGGGGATGGGGACTATGGGAGAGATCGAAACGCTGTTGCTGTTGGGTAACAGGCGAGGAACTTCCCCGGCCG
TGCTCTCAGTGCCCGGCGCTGTGGAGGAGCAGTCCGCGG



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 2.6 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme SacII.
Predicted to produce a 2 kb band using primers LC24 LC18.
IPCR Notes:

Product was used in PCR again using primers JGp2 LC45, producing a 2 kb band.


Sequencing

IPCR product was processed by precipitation.
Sequencing reaction was performed using primers JSR05 and JSR04.
Sequencing Notes: Two Reads

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