B.W06.1071 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

5', 3', Adjacent, Distal

• 5' or 3' refers to the direction of flanking Ds sequence
• Adjacent or Distal refers to the proximity of flanking Ds sequence to the Ds insertion point
• See diagram below right showing all 4 locations

Placement Scores:

• SP = Single Placement (single chromosomal location);
• DP = Dual Placement (two possible chromosomal locations, not resolved);
• MNP - Multiple Locations, Not Placed (too ambiguous to call)
• NGH = No Good Hits, Not Placed

Quality refers to the Megablast % identity/coverage score:

• HQ (high quality): > = 95% coverage and >=95% identity with no large gaps.
• MQ1 (medium quality 1) > 90% < 95% coverage and > = 95% identity with no large gaps.
• MQ2 (medium quality 2) > 90% coverage and 95% cumulative identity, with one or more large gap openings in alignment
• MQ3 (medium quality 3) > = 95% identity over at least 200 bp of sequence
• MQ4 (medium quality 4) >90% identity over at least 200 bp.

Alt refers to the alternate locations under DP:

• If exactly two locations of HQ (high quality) exist, both are reported as DP