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B.S07.0271R (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 169809456 (B.S07.0271R_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|169809456 (219 bp)

>gi|169809456|gb|ET438031|B.S07.0271R
CCAGCATCTGGTCCATCGTTTATAGTAGCTAGTAGCTACTACCACCACCAACCAGTACGTTCTTGAGAATTAATGTACGCGTGTGCGAAGATCGATCGAG
CTGAGCGGATGTGGATGCGATGTGGGATTGGATATATATATAAATATGATCATCACGGTGGTGGTGGTGGTGGCCCTTATATATAGCGCGCGGGAGGCAA
CGCGCGTGATGGCCCGCGG



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for linked transpositions.
Identified as Southern band of 1.6 kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme SacII.
Predicted to produce a 0.6 kb band using primers LC24 LC18.
IPCR Notes:

Product was used in PCR again using primers JGp2 LC45, producing a 0.5 kb band.


Sequencing

IPCR product was processed by ethanol precipitation.
Sequencing reaction was performed using primers JSR05 and JSR04.
Sequencing Notes: none

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