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B.S05.0813A (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr1:8,429,455 GRMZM2G152172_T01 0.3 kb from 5` end gi|133823082 (B.S05.0813A_JGp3) 3`Adj
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Redundancy Group: RS.0099

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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 133823082 (B.S05.0813A_JGp3) Blast@ZmGDB

Show FASTA Sequence for gi|133823082 (412 bp)

>gi|133823082|gb|EI414112|B.S05.0813A
GTTTTGGAACCTTATTCGTGGTAAATATATGGCATACGACAGTACAGTACGTGTTGGTGGTGCATGAGCCTGCTGATGGATCGGTGCGTGCATGGAGCCA
GCCAGACGCGGCAGCTGACTGGCGAGGGAGGTCACAAGCAGTGGCGCGGCGTGCGCATGAGTCACGTTTCGCCCCTCGCGTGCCCGGCCTGAGCCCGAGG
GCTCGCCGCCAGTTACTAGTGGGCTAGTATAAACCTGCACCGGCAGCCGGCCAGCCAGGCACCAGCACCACCAGAGTCCAGAGCAGCAGCTCCTTCATGA
CGAGCTTTCTCCAGCTCTGAGCGCGCGGAGGGATCGGAGAGGCGCGATGAACGGGGGAGAAGGAGAAGGAGGAGGAGCGGCAGCCGGCGGGGCCGGGGCC
GGGACACCGCGG



Genetics

Line was produced using r1 as a donor.
Ds element donor under selection for unlinked transpositions.
Identified as Southern band of 2 kb.
Southern Notes:

View Image: Southern Blot showing B.S05.0813A
(Click image for larger version)

Southern Image

Inverse PCR

Genomic DNA digestion performed using the restriction enzyme SacII.
Predicted to produce a 1 kb band using primers LC24 LC18.
IPCR Notes:

Product was used in PCR again using primers JGp2 LC45, producing a 1 kb band.


Sequencing

IPCR product was processed by gel purified-freeze dried.
Sequencing reaction was performed using primers JGp3 and LC42.
Sequencing Notes: Coll. B.S05.0813B

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