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Ac.mon00236 (Ds Insertion Line) Note: Outdated assembly! Go to RefGen_v2 Admin

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Ds location and closest gene Order Seed View all Ds Insertions

Alt	Est. Ds Insertion Site	Closest Maize Gene	Ds Distance from Gene	All Ds near gene	Flanking sequence (fDs)	Sequence Type
1	Chr9:23,423,742	GRMZM2G121333_T01	12.5 kb from 5` end		gi\|54292152 (Ac.mon00236_JSR03)	5`Adj

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Flanking Ds (fDs) placement in the B73 RefGen_v1 Assembly:

Ac.mon00236	fDs Placement				fDs Placement alternative (if any)
Ds Element in relation to fDs -⟩
Sequence Type -⟩	5' Distal	5' Adjacent	3' Adjacent	3' Distal	5' Distal	5' Adjacent	3' Adjacent	3' Distal
fDs CloneID		Ac.mon00236_JSR03
flanking Ds (fDs) gi (Click gi to view primary placement data)		54292152
fDs Acc		AY559188
fDs Seq Length		479
Single/Dual Placement		SP
Placement Quality		MQ3
Likely Ds Insertion Point Click to view at ZmGDB		Chr9:23,423,742
Closest Gene (Click to view) Ds Distance (red=inside gene)		GRMZM2G121333_T01 (12.5 kb from 5' end)
Gene BLASTp top hit Click to view UniRef record		UniRef90 blastp 6-15-2010: Q5NKT2_SORBI Putative uncharacterized protein Sb10g002090 n=1 (Sorghum bicolor) Exp=0...
BLAST fDs @ZmGDB (Zm-genome masked)		Blast@ZmGDB
Chr		9
fDs Left Coord		23,423,742
fDs Right Coord		23,424,156
fDs Orientation		+

Click for explanation of location, placement (SP, DP) and quality (HQ, MQ1, etc.)

How to request seedstock containing this insertion (click for details)

Flanking genomic DNA sequence

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 54292152 (Ac.mon00236_JSR03) Blast@ZmGDB

Show FASTA Sequence for gi|54292152 (479 bp)

Genetics

Line was produced using as a donor.
Ds element donor under selection for transpositions.
Identified as Southern band of kb.
Southern Notes:

Inverse PCR

Genomic DNA digestion performed using the restriction enzyme .
Predicted to produce a kb band using primers .
IPCR Notes:

Sequencing

IPCR product was processed by .
Sequencing reaction was performed using primers JSR03 and .
Sequencing Notes:

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