DS LOGO

Ac.bti00228 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr6:91,278,581 GRMZM2G146140_T01 -2 kb from 5` end gi|54289513 (Ac.bti00228_JSR03) 5`Adj
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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 54289513 (Ac.bti00228_JSR03) Blast@ZmGDB

Show FASTA Sequence for gi|54289513 (1992 bp)

>gi|54289513|gb|AY618475|Ac.bti00228
CTCCCATCATTGCTAGCGCTAACCGTTATTTATCTGGATGGTGGGCGAGTCTTCTTAATCACCATGGCAGACTTACTCTTGTTAATGCAGTCTTGGACAG
CTTGCCTGTTTATGCAATGGGGGCTCTCGCCCTCCCTCCAGGCGTGATTGAGGCCATCGACTCTAGACGTCGGGCTTTCCTATGGGCCGGGGAAGAAACA
GTCTCTGGTGCGAAGTGTCTTGTGAATTKGGAACGGGCATGTCTCCCGAGAAAAGATGGCGGACTGGGTGTTCGTGATCTCCGCTTGCAGAATACCTGTC
TGCTACTGAAGCTCCTCCATCGTGGACACAACTCACGTGACTCAGCTTGGGCCCGCTGGTTAGAAGTGGAATTTGGAGGCCCCATGTCTGCTCCAGACAA
CACGGCAGCTGGTACTCATTGGGCTGCCCTCCGACGCCTTCTGCCTGACTATCGCTTACTTACCACGGTGGAGGTGGGCGATGGTCGCTCTACAGCCTTT
TGGCACGACTGCTGGTTGTCTACGGGGCCTCTTGTGGATGCCAYGCCGGCGCTCTACTCGCACGCCCGACGCAAGGAAACCTCGGTCTGCAATGTCCTGT
CTACAGCCCTTCGCATTGCATTTGTCCCTCGCCTCTCCACCGTGGCTGCTAGAGAATTGGAGCAGCTGGAGGCGCTCCTTGCTGGTGTATCCTTGTCTGC
GACTCCTGATCTCCGGTTGTGCCCGTGGGAAGACATGGCACATAAACTCAATTCATCGACTGTCTACCAAGCTGTTGTGACTAATGGCCAGGGATGCGAG
TACTACAAGTTTATCTGGGAAATCCGTGCTCCCCCTAAAGTTAAGTTCTTTGGCTGGCTATTGGTGCAAAACAGGATTCAAACCAAAGATAATCTTCTCA
AAAAACACTGTGTGGAGAATGATATCTGTGAACTTTGCTTGTCGGCCGTGGAGAGCTCGGTGCATCTGATTTCGGGCTGCCCTTTTGCTGTTGGCTTCTG
GGCGCGACTAGGAATATCTCTCACAGACGAAGACGCGTCACATCTTTGGTGCGTTCGATCCCCAGGTCACTTGCCTGCTGCACACTTTAATGTTTTTCTC
CTCTTGTGTTGCTGGCGTCTCTGGAAGCACCGCCACGATGTTGTTTTTCGCGCTCTCTCTCTCCGAGCTATGAGCGTCTTATGGCTGGTTGTCGTGATGA
TGCAGAATTGTGGTCTTGTAGGCTCCCCTCTAATGATCGTTATGTAACCCTCGCATGGGTTTCGATATTCTCTTCGTTAATCCCCTCTGTAACAACTTAA
CCGACATATAACTAACTCATGTATGAGCTTTTATGTTGGCAATGAAAAGGTGGGGTATCTCGCCCCCCCCCCCCCCCCCGAATTTCTCAAAAAAAAAGAA
ACCTACCGGCCCCTAACTACGACGGAGACCAAACGCACTGTTCCTCTGTTTGATCTTGTTGTATTTCCTCCACAGCAGAGACGACCTTGAACTGAAGGTC
TGTGATGCAAAAAGGGACACCAAGGTCTGTGATGCAGCTTGCTTTCTCAGCAAGAATTTCCAGCTTCTCAGAACTGAAGGGGAAGGCCTCCCACCATGCG
TCAAAACCATGGTCGTGGATTATTTATTTCCGCAACATTGTTGAACTAGTGTTGGAAGCGTCATAATAAGCTCARAGTGACGAACCAGACTCACACACTT
GTTGTCTGTCACTTCCRGGAATTGCAAAGAAAGTATAGTCTCGTATGTAACTGAGCTTACCCACGGCGAGCTCGCAGCCCGCTTCGTCGTGTAYTGCAGC
TAARGTCGATTCTTCAAAGCTTCACCCGTGCAACAGGTCTGGATATAAACTACAACAAAAGTACACTGGTTCCAATGCATGTGCCTGCTCCTGATGTCAC
TCGATTCGTTAACATTCTTGGGTGTGCAGAAGGTGCGTTCCCGCAGACCTACCTTGGTCTCCCTCTCTCTAATGAGAAATTGAACCCTGCAG



Genetics

Line was produced using as a donor.
Ds element donor under selection for transpositions.
Identified as Southern band of kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme .
Predicted to produce a kb band using primers .
IPCR Notes:


Sequencing

IPCR product was processed by .
Sequencing reaction was performed using primers JSR03 and .
Sequencing Notes:

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