DS LOGO

Ac.bti00220 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr3:139,981,236 GRMZM2G164502_T01 -0.4 kb from 5` end gi|54292181 (Ac.bti00220_JSR03) 5`Adj
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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

5' Adjacent fDs: 54292181 (Ac.bti00220_JSR03) Blast@ZmGDB

Show FASTA Sequence for gi|54292181 (1874 bp)

>gi|54292181|gb|AY559218|Ac.bti00220
GCAGCAGCAGCAGCAAGCACTGCTACACGCTGCACGTCCGCACCCGCACCCGCTACCTCGTCCGCGCCACCTTCCTGTACGGCAACTTCGACAGCAGCAA
CGTGTTCCCGGAGTTCGATCTCTACCTTGGCGCCTCCCACTGGTCCACCATCGTCATCTACGACGACTCCAAGGTGGTCACCAGGGAGGCCGTCGTCCTC
GCCGCCGACCCCGCCCTCAGCGTCTGCCTGTCGTCCACGGGGACGGGGACGGGGACGCCCTTCATCTCCACGCTGGAGCTCCGGCAGCTGAACGGCTCCC
TCTACTACACCGACTACGAGGCGGACGCCTTCCTGGCCCTCTCCGCCAGGATCAACTTCGGCGCGCCCACCGCCGACCCCGTCAGGTACCCGGACGACCC
CTACGACAGGATCTGGGAGTCGGACATGGTGAGGAGGGCCAACTACCTGGTGGACGTGGCAGCCGGCACCGTCAACGTCTCCACCGACAGGCCCGTCTTC
GTGGCCGGCAGCGAGAGGCCCCCGCAGAAGGTGATGCAGACGGCGGTGGTCGGGTCGCTGGGCGAGCTCACCTATCGCCTCGACCTCCCAGGCTTCCCGG
GCAACGGCTGGGCCTTCTCGTACCTGGCGGAGATCGAGGAGTTCTTGGTCCCGGAGACGCGCAAGTTCAAGCTCTACATCCCGGGGCTGGCGGACGTGAG
CAAGCCCACGGTGGACATTGGCGAGAACGCGCCGGGCAAGTACCGGCTCTACGAGCCAGGCTTCCCCAACATCTCGCTGCCTTTCGTCCTGTCCTTGGCG
CTGAGGAAGACCAACGATTCGTCCAAGGGGCCAATTCTCAACGCCTTGGAGATATACAAGTACATGCACATGGAACTCGGCTCACCGGATGGACCTGTGA
TGGCGACCCTGAGCCTGGCCCTGGCGTCGTCGTCGTCCTTGGCTGATTGGGCGATGGAAGGCGGTGATCCCTGCCTACCTTCACCCTGGTCCTGGGTCAA
GTGCAATTCAGAAGCGCAACCAAGAGTAGTTTCCATGTACAGCTAATTCCTATTAATTCATCTCTGAGGAGCTAATAAGCCACGCACTGACCTAGCTTGG
TGGTTGTTCGAATCTGCAGAAATCTTTCAGGAAAGAACTTGACAGGGAGCATACCACCCCAAGTAGCGGATTTGCCCTGCTTAGCAGAGATGTATGTATG
TATGTAAGCTAGCCTAGCATTGATTGTCATTTGAACCTGTCTGTCAGCAGCATTTCAATTCTCTTTGCAGCGGATTTGCTAATAACATGTTGACGGGCCC
AATTCCTGATCTCAGTGGCTCCTCCAACCTATCCAYCATGTGAGCATTTCAATTCTTTTGCTGCTCATCATTGTCCTCGTCTCCTTTAATTTCCTCGTTA
AAATGCATGCATGCAGTCATCTTGAGAACAACCAACTGACCGGAAATGTTCCTTCCTACTTTGGCAGCTTGCCAAAACTGAGCGAGCTGTAAGTATAATA
GTATAGTGTACTTCCTTCCTTTATATATATTGGTTGAATTAAGATTCCTTCAATTCACCAATACAACAACTGTTCAGGTACCTTGAGAACAACCGGTTGT
CTGGACCCATTCCCAAGGCTCTGCTAAGTCGAAGCATTATTTTCAAGTTCAGTTTCTTTCTKGATCAATAATATCTTCAGTTTCTTTCTTGATCTGATAT
CATCTCTGCTAACATGTAAACAAATCTTCAGCTACTCTGGGAACGTGTACCTTGGAACAGCAGGGAAGCAAAAGARGAAGCATGTCATCATCATTATATC
TGCTCTGCTTGGAGCATCCTTGCTACTTGCAGCCGCCCTCTGTTGCTATATGCTGACACGCAAGGCCATGAATT



Genetics

Line was produced using as a donor.
Ds element donor under selection for transpositions.
Identified as Southern band of kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme .
Predicted to produce a kb band using primers .
IPCR Notes:


Sequencing

IPCR product was processed by .
Sequencing reaction was performed using primers JSR03 and .
Sequencing Notes:

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