DS LOGO

Ac.bti00207 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr4:177,739,470 GRMZM2G015983_T01 0.2 kb from 5` end gi|54289514 (Ac.bti00207_JSR05) 3`Adj
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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 54289514 (Ac.bti00207_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|54289514 (2556 bp)

>gi|54289514|gb|AY618476|Ac.bti00207
CTCGTGGGTTGTCACTGTGGTGGGAACGAGGAACAATTTCCTATAGCTTCGGCCGGTTCCATGGAAAAGTCTCTGCTCTTTCTAGCCTAACTGTAACTTT
CTTTTAGCACTTGGTATTCTCGCCTCCAAGGCTCCAAAGTTCAGGTTTTGACCGTRTTTTCTTCCGAAGCAATAGGTGGTAATCGTCAGATCGAACTGTT
CGTTAAAATTCTTGTAAAATGGGTACGACGATATGTATTCAAATTGCTTGCCCGCTGTCTTCCTTTCGCGCTTCTCATCTTGCTGCAGAGAGAGGACGCA
CTTTCCTTTGCATGCAGGGAATTGCATCCATAGCTCCGTCTCCGAACCGCGATCACGGCCATGCCATGCTGCCGTGCGTGCGCTAGGGTTTCTTCCTTCG
CTTCCACTCCCCGCGGCCCGCACATGTTTTTTGTTTTCCATTGGCAGGACTCCCACATGGTTTTTTGGTAGTTTGCGACGCCCTGCTGCACCTCTGATGA
CCTGTCACTAGTTTCCGTGAGACGGGGGGGCACAGGACAGGACGGCTTCCCGCGAGGACATGTGGTCAGATTCTTCCGAGAAGGTTTTTTTTTACTACTT
CGTTTTCATGGGCTGCGGAGTTGCAGGCCGGCCAGGGATCAGCTAGCAGCGTCGGCTCTATCATTGGAGCTTAATTCAACGGTGGTAGGTAGGTGGAGTA
ATGGAATGGAATGGGTACGTACGCGCATCTGAAATTCTGAATCGTTCAACGCCAACGAGCTGCGGAAATGTCGTAATCAACCCTAGTGATGTACTACGTA
CGYATCGTATTCGTACATTTACTTGTTGAATACTTCAAAAACTCGTCCGTCGCCGTCACCCGTCACCATCAGGGAGAAGGCCAGAGCTCAGGCTGCGCAT
AGGTGTCGTCGACCAGAGACATTGCACCATCATCACATCACAGATTAATGCAGCACAGAAGCCGGAAGTGCGGAGACTGTCGYCTCCAGAAGCTGGAGAT
CTACGATCTTCTGCGCTCGATCTTCAGAGGAGGGAGGATCTGTGACCTGCCATATGTCCACGAGAATGCAAGGAGACACGTGCAACAGCCACTGCTGTCC
AGTGCCGCATCTATCACCACCATCCCTCCTCGTAGGACGCCCTATTTTCCTAAGCTGCAGCCTGCAGAGGAGACTGATGAAGAATAATGTTCTTTTATTA
TTAGAGCCAGTAGCCGGACGTCAACGCGAGCGCAACTAACGCAGACGGCAGACTGGCCGGCGGTTCCACCAAGTGTGCCTGTCACCGGCCGGGCAGCAAG
TCCGCGTGAACCAGCGTAATTAGTTCAGCTCGTACGCGATCACTGCACGCGCCATCGGATGCTGCCGCCGTGGGTTCATCATCGTGTCTCGTCGGTGAGC
GATGGACACGGAGGAGTGGAGGTGGAGCTATTACCCATGACTCCCGCTCCCACCGATTTTACTGTCTGGTTACGGGGAGCGTGACACGACCGCATCATGC
CGCTGCACAAGCTAGTGCCATCTGCGCACATGGCTTGCCGCTTGGCGCCTGGCGACTTCTACTGTGCGCAGCATGGACAGAAGAGGTGGACGGATACCCT
CCCGTCGCCACGCGCGTCGGTGACTACTGACGCGAACAAGGYTTACTTGTGTCTGTGGTCCGAATTAATGACGAATAATTATCAGCAACGATTCTAGACT
CTCACCTCTTGTGTAAGGAGATCCGCAACGATTTTGTCTGTCGATTATGTCTAAGAGCTGTATTGTTTCATCTGAGCAAGGGTCACAGGACCGGCCTATA
GCTTTTGGTAGGCTTTAAATTCAGTTCGTGCTCTTGCTGATTTTCTGAAGTCTTTTGTCCTTGAGTTCTTCGTCTCTTTGTTCTTGTTGATTTGAGAAGA
ACCATCGAGTTCTTGTGTTTTCATGTGTTTTTTGGGATTTGTTGGTGTGCGAAATACACTCTTAGAATCACTACTTACGTCCTATTGGCGTAGTCTGTGA
TTGTGATCTTAGCCGATCTTGTTTTGAGTTCGTTTTAGAAAACATCAACAAAACACAATTCTATTCGTATTTTTAGATCCGTGGTCGATTCACAAGTTCG
ATTGGGCTCAAAATTTGTCCAGGTGTGTGTAAGATTTTATCTCAATCGGAGCTCGTTTGGTTCAGTTTCGTATTCGAGAACTGAATTTCGTGCTGCTGAA
ACGAGCACTTGTTCGTGACACGGTTTTGGACGGATTCAGATTTTTTGATGTCCGATTTGCGATGCGTTTGTTTTGCTAGTCTCGTGTGAGTATTAGAAAT
GTTTTGTTTTGAAATTATAAGATTACTGTTTTGCTGATGTAATTTTGGTTTGATCGCTTACTTTATTTTAATTGAAGTGAGGTGACCATATTTAATGTTT
GGTAGCTGGTTCGAGTTTTGAAGAAACTTGGCTGGACCGCGTGAAGCACGGTGCCGAGAGCCGTTAAGCCGTTTGCAGCAGTTTATCTAAAGTTTACTCA
AAAGAACTGTTTTGTACTGCTACTAAAGTCGAGTTTAAATGAGCGGAGGCAGAATT



Genetics

Line was produced using as a donor.
Ds element donor under selection for transpositions.
Identified as Southern band of kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme .
Predicted to produce a kb band using primers .
IPCR Notes:


Sequencing

IPCR product was processed by .
Sequencing reaction was performed using primers JSR05 and .
Sequencing Notes:

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