DS LOGO

Ac.bti00191 (Ds Insertion Line)    Note: Outdated assembly! Go to RefGen_v2         Admin

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Alt Est. Ds Insertion Site Closest Maize Gene
Ds Distance from Gene All Ds near gene Flanking sequence (fDs)
Sequence Type
1 Chr2:3,585,258 GRMZM2G365162_T01 -0.1 kb from 5` end gi|54292167 (Ac.bti00191_JSR05) 3`Adj
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Flanking genomic DNA sequence

diagram of ds insertion

W22 flanking DNA sequence(s) (fDs) listed under Putative Location in B73 Genome (RefGen_v1) were derived from 5' or 3' end of Ds-containing fragment, using primers either adjacent (5a, 3a) or distal (5d, 3d) to the insertion site. Directionality is as shown in the diagram. For more information see AcDs Help. See below for FASTA sequence and BLAST links:

3' Adjacent fDs: 54292167 (Ac.bti00191_JSR05) Blast@ZmGDB

Show FASTA Sequence for gi|54292167 (1434 bp)

>gi|54292167|gb|AY559204|Ac.bti00191
GAGAGGTGGCTTGGACTTGGAGGCAGACTTGCGGAGGGGAAGGTAGCTAGGCTTTATAGGGAGGAGGGGCCCCTYGGCGACCCTTTGCCTTTCGGATCGT
CCAGTTGGCCCGGTATAAAATTATGCTGAAAAGGATGGATTGTTATTCTTCTATTCTTCTATTCTACTACTCTCTGTAATTTGACATTGTCTATGTCTTT
TTTTTTTTTGAGAGAGAGAGGGGTTGCAGGATGGGCGTGAAGAACTGGGCAATGGAGACAAGAACTCACAAGATGGTTATACAAAACTACATCCTGTGTT
TATAGAACTATTAGAGAAATTACAGTTTGTTACTCTCAAACTTATTTAGCRACTTGTTAAATCTACGTAGAGAGCTAAAATTTACATAACTATTGGAGTT
GCTCTTAGAGTTGCGCTTAACCATGTCGTGACTCTCATTCAGAGTGGAACTGCACACATGTATAAACGGAGGAAATAAAAGTCCATCCATCTAACAAAAG
TATTTTTTTCCCTGAACTATTATTGGTAAGCGCACATGCTTAACGTTTTAAAACCGCACCGTCAAATGCCTGCCTGCCTGCCTAAACTATGCAGCTTGCA
CACCCTTGTGCTACCAAGAAATGGAGCGCACAAACTTCCCTGCCTCTTGCGCATCCGCAGATCAGCAGATGCCATTTTCGCCGCTGCACTTGTTCAACTC
GCTCCCCTCAAGTTGTGCATGCACGGGTGATAGGTGCACCGTGCAGGGTACACCACGCTGCTCGTGTTCTTCCTCAAACTCGGAATACGAGCGCAGTGGC
GGGGACAAGTTTTTGTCACTTTGCAAACGTGCGGAACCAAACGAAGCTCGCTGTTGCAGTGTTTTCTTCGTGTCGCGTCGGACAGTGAACAGAGCAGACC
GGAGTAGGTAGACAGTGAAGAGAGCACTGCACAACATTGTGTCCGTAGCATCGCATCGTGGCCGGGGCCAGCAGTCGCGTCACACCATCGGCGGCGGTGT
CGAGAGGCCAGGCGAGGTGCCCCCCTCTCTCYGATCTCTCCGAACTCTGACAGGTGAGGTGAGCGAGACGAGTGGCGRCCCAGRGTGTGGGAAGTGCGCT
GCTGCTGGTGCATGTGACCGCAGCCGGGCGCGCATGTGCACGAACCAGTGGCCTGTCTGGTCCGGCCGATCTCTCCTCGGATTTTTGCGCGCAGTTCTCT
GCCGGGGCCCGTGGCTTTCGAGGATCGCCGGGAAGATTTCGCGGCACGCCCAGCAGCATCCAGCAAAACTTGCCGGTAACCCCGTGTGTCCTGGTGTGGT
GGCTATGCAACGGAGCTAGGTGCGGTGCAGCAGAGAGAGAGGCTTCGTACTAGCATGTTTCCCTGGCTGAACACTTTTTGTCAAACTCTCTTTCCGCGTG
GAGTAGGAGTAGCGTCCAAGCTTGCAAAACTGCA



Genetics

Line was produced using as a donor.
Ds element donor under selection for transpositions.
Identified as Southern band of kb.
Southern Notes:


Inverse PCR

Genomic DNA digestion performed using the restriction enzyme .
Predicted to produce a kb band using primers .
IPCR Notes:


Sequencing

IPCR product was processed by .
Sequencing reaction was performed using primers JSR05 and .
Sequencing Notes:

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