Genome / Gene Models
The most current genome assembly displayed at MtGDB is version
Mt 2.0;
gene models are from
Mt 2.0.
More about the genome...
Medicago truncatula genome at MtGDB
| Chr |
Length (bp) |
Gene Models |
| 1 | 31,270,187 | 4,468 |
| 2 | 27,869,060 | 4,047 |
| 3 | 38,177,656 | 5,444 |
| 4 | 39,361,788 | 5,592 |
| 5 | 37,289,045 | 6,277 |
| 6 | 19,993,879 | 2,496 |
| 7 | 32,528,341 | 4,452 |
| 8 | 35,250,466 | 4,637 |
| 9 | 16,948,850 | 1,431 |
| Total: |
|---|
| 9 | 278,689,272 | 38,844 |
Alignments to Genome
MtGDB displays high quality spliced alignments for EST, cDNA, PUT, and model species proteins.
More about alignments...
- Transcript sequences displayed at MtGDB were aligned to genomic sequence using GeneSeqer, which performs a consensus spliced alignment on ESTs and cDNA, providing both cognate and non-cognate alignments for improved gene prediction (Brendel et al., 2004).
- Related-species predicted proteins were aligned to genomic sequence using GenomeThreader (Gremme et al., 2005).
- Microarray probes (if any) were matched to the PUT assembly sequence using Vmatch and then coordinately transfered to genomic location.
Search / Download
Search or download any splice-aligned sequences, gene models, or genomic sequence from MtGDB. The entire MtGDB dataset can also be downloaded.
More about search / download...
| Resource |
Description |
| Search by ID / Keyword |
Search for any MtGDBgene or aligned sequence by ID or keyword, and download complete sequence or flanking 5' or 3' regions, exons, or introns (FASTA format).
|
| Download Region Data |
For any specified genomic region, download genomic DNA (FASTA format), all aligned/computed transcripts or proteins (FASTA format), or all genome annotations (GenBank, GFF3 or EMBL format).
|
| Download All |
Download bzip2 files and MySQL tables representing complete MtGDB dataset |
Note: sequences displayed here may not reflect the latest Medicago truncatula sequence count at PlantGDB, which can be viewed on our download page.
Tools
Sequence analysis tools accessed from the
Genome Context View menu use the current genomic region as input. Or, to access the same tools without a genomic context, use MtGDB left menubar Tools links.
More about tools...
Online Analytical Tools - Description
| Name |
Description |
Source/Version |
| BLAST |
Use BLASTn, BLASTp, tBLASTn and tBLASTx to match a query sequence to MtGDB sequences.
BLAST-able datasets include Medicago truncatula genomic DNA, splice-aligned transcripts and proteins, gene models, and other aligned sequences. |
NCBI Blastall 2.2.18 |
| GeneSeqer |
Spliced alignment of user-specified transcript datasets to genomic DNA from any region in MtGDB. User can specify splice site model and stringency level. |
Brendel Group (Oct 2008) |
| GenomeThreader |
Spliced alignment of user-specified protein datasets to genomic DNA from any region in MtGDB. User can specify splice site model and stringency level. |
GenomeThreader Home Page |
Gene Structure Annotation
Help annotate the
Medicago truncatula genome using our yrGATE gene structure annotation tool (
see Overview), aided by
GAEVAL tables that flag problematic gene models.
Video demo: QuickTime
Flash
Custom Track Display
Distributed Annotation Service (DAS) (
see Overview) is available for displaying remotely served genome alignments at MtGDB, as well as for displaying selected MtGDB tracks on a different genome browser (e.g. Ensembl, GBrowse).
How to use DAS...
To add a DAS track to MtGDB using our DAS client, first select desired genome coordinates above and click "Go", then select menu item "Configure -> Add Track" and paste in the appropriate DAS URL.
To view MtGDB tracks in another genome browser (e.g. Ensembl, Gbrowse) using our DAS server, first find the appropriate xDAS Server URL on our DAS overview page, then follow instructions on the remote browser website for adding the remote track. NOTE: DAS service available for certain tracks only.
For more details, refer to DAS section in our Genome Browser help pages.
Additional custom track display features are under development.
Annotated Protein Alignments
A searchable table of splice-aligned, annotated protein models from one or more related species. Select from the left menu under Gene Annotations -> Splice-Aligned Proteins.
Support
Genome browser video demo: QuickTime
Flash
For help pages and tutorials focusing on genome browser features and gene annotation, see side bar.